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EC Number
Purification (Commentary)
; nickel affinity chromatography, recombinant CobS
solubilisation and extraction from Escherichia coli C41(DE3) membranes in presence of DHPC (15 mM) following overexpression with hexa-His-tagged from pET15b plasmid carrying CobS (pCobS5 (cobS+))
Superose 12 HR, Phenyl-Superose, partially purified
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