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EC Number
Purification (Commentary)
after expression in Escherichia coli with N-terminal His-tag
extracellular enzyme from culture medium by anion exchange chromatography
extracellular enzyme from culture medium by several chromatographic steps including one affinity based step
native enzyme 13.1fold by ethanol precipitation, gel filtration, and anion exchange chromatography
native extracellular enzyme 5.6fold from culture supernatant by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration
recombinant enzyme
recombinant enzyme from Bacillus subtilis culture medium by ammonium sulfate fractionation and hydrophobic interaction chromatography to homogeneity
recombinant enzyme from Escherichia coli strain BL21(DE3)
wild-type and mutant enzymes
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