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Search Purification (Commentary)

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EC Number
; recombinant domain I and recombinant E155W and Y171W mutant domain III
; recombinant protein
; the recombinant enzyme is purified by pre-extraction of the membranes with sodium cholate and Triton X-100, solubilization of the enzyme with sodium deoxycholate in the presence of 1 M potassium chloride, and centrifugation through a 1.1 M sucrose solution. The wild type enzyme is purified by phenyl Sepharose column chromatography, DEAE-Bio-Gel A column chromatography, and NAD+ agarose column chromatography
DEAE-Sepharose column chromatography and hydroxyapatite column chromatography
fusion protein using His-tag and on calmodulin-sepharose
His-tagged H91E mutant enzyme
Ni-NTA resin column chromatography
phenyl-Sepharose column chromatography and DEAE-Trisacryl M column chromatography
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