EC Number |
---|
1.1.1.169 | anion-exchange chromatography, gel filtration chromatography |
1.1.1.169 | chromatography |
1.1.1.169 | FPLC, anion exchange chromatography, gel filtration |
1.1.1.169 | gel chromatography |
1.1.1.169 | homogeneity in a yield of 20-60 mg from 3-6 g of cells |
1.1.1.169 | native wild-type enzyme by anion exchange and adsorption chromatography, and gel filtration, recombinant His6-tagged enzyme by nickel affinity chromatography |
1.1.1.169 | on nickel-nitrilotriacetic acid resin and by gel filtration, more than 98% pure |
1.1.1.169 | recombinant C-terminally His-tagged wild-type and mutant enzymes |
1.1.1.169 | recombinant enzyme from Escherichia coli by heat treatment, anion-exchange chromatography, and gel filtration |
1.1.1.169 | recombinant wild-type and mutant enzymes from Escherichia coli strain Rosetta2 (DE3) pLysS by nickel affinity chromatography, avidin affinity chromatography, and dialysis |