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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the reaction diagram Show all sequences 1.1.1.325native enzyme 533fold from cytosol by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration followed by affinity chromatography, another different step of anion exchange chromatography, and ultrafiltration
Display the reaction diagram Show all sequences 1.1.1.325recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)
Display the reaction diagram Show all sequences 1.1.1.325recombinant His-tagged wild-type and selenomethionine-substituted enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, anion exchange chromatography, and gel filtration
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