EC Number |
Reference |
---|
1.11.1.12 | - |
394864, 394867, 394868, 394869, 394873, 394876, 394878, 659647, 672372, 687471 |
1.11.1.12 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Sephadex G-50 gel filtration, and HiTrap SP column chromatography |
701060 |
1.11.1.12 | by Ni2+-nitrilotriacetic acid Sepharose |
698037 |
1.11.1.12 | by nickel affinity chromatography |
696173 |
1.11.1.12 | from cytosol and mitochondria |
394880 |
1.11.1.12 | large-scale, wild-type and mutant SeC46C from baculovirus/insect cell expression system and mutant SeC46C from expression in Escherichia coli as His-tagged fusion protein |
394879 |
1.11.1.12 | on a Ni-NTA column, further purified by gel filtration |
701118 |
1.11.1.12 | partially |
394866, 394882 |
1.11.1.12 | partially from testis and to homogenity from sperm as recombinant enzyme expressed in Escherichia coli and as wild-type enzyme |
394877 |
1.11.1.12 | Percoll step gradient and nickel affinity chromatography |
676554 |