EC Number   |
Reference |
|---|
    1.13.11.6 | - |
439364, 439365, 439369, 439370, 439371, 672036, 672037 |
| 1.13.11.6 | bovine kidney homogenized in 0.1 M potassium dihydrogen phosphate buffer with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, centrifuged, protein fraction of supernatant that precipitates from 34-62% saturated (NH4)2SO4 is resuspended in 5 mM potassium dihydrogen phosphate buffer with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, dialyzed against same buffer containing protease inhibitor PMSF (0.1 mM), applied to DEAE Sephadex A-50 column, fractions with enzyme activity pooled and concentrated by precipitation with 80%-saturated (NH4)2SO4, dialyzed against 10 mM Tris-HCl with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, applied to Blue-Sepharose CL-4B column, concentration of active fractions by ultrafiltration through YM-10 membrane |
696784 |
| 1.13.11.6 | liver |
439376 |
| 1.13.11.6 | pure liver enzyme, partielly purified brain enzyme |
439367 |
| 1.13.11.6 | recombinant |
677020 |
| 1.13.11.6 | recombinant His6-MBP-tagged enzyme from Escherichia coli by nickel affinity chromatography and cleavage of the His-tag by TEV protease, elimination of the tags by nickel affinity and amylose affinity chromatography, te final step is gel filtration |
741516 |
| 1.13.11.6 | succesive size exclusion and affinity columns |
658927 |
