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Search Purification (Commentary)

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Results 1 - 5 of 5
EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.66-
Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.66ligandation with 4-hydroxybenzoate prevents the enzyme from irreversible inactivation
Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.66recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.66recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag removal by rTEV protease cleavage, over 95% purity. No enzyme activity can be detected with purified YaiA, even when it is purified in the presence of 4-hydrobenzoate
Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.66using Ni-NTA chromatography
Results 1 - 5 of 5