EC Number |
---|
1.14.13.1 | - |
1.14.13.1 | affinity chromatography |
1.14.13.1 | cells grown on 1-hydroxy-2-naphthoate |
1.14.13.1 | DEAE-Toyopearl 650M column chromatography, phenyl Sepharose CL6B column chromatography, and Sephacryl gel filtration |
1.14.13.1 | HisTrap column chromatography |
1.14.13.1 | native enzyme from 5-chlorosalicylate-grown cells by anion exchange chromatography and gel filtration |
1.14.13.1 | Ni-NTA column chromatography and Superdex 75 gel filtration |
1.14.13.1 | Ni-NTA His Bind resin column chromatography |
1.14.13.1 | oxygenase component PhnII |
1.14.13.1 | recombinant expression of His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography, tag cleavage and a second step of nickel affinity chromatography |