EC Number |
---|
1.14.13.24 | - |
1.14.13.24 | 98% homogeneity |
1.14.13.24 | DEAE-Sepharose column chromatography and Sephadex G-25 gel filtration |
1.14.13.24 | homogeneity |
1.14.13.24 | Ni-NTA agarose column chromatography and Q-Sepharose column chromatography |
1.14.13.24 | recombinant His-tagged NarX from Escherichia coli strain Rosetta (DE3) pLysS by nickel affinity chromatography to homogeneity |
1.14.13.24 | recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
1.14.13.24 | recombinant N-terminally His-tagged Ncgl2923 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity |
1.14.13.24 | recombinnat His-tagged enzyme from Escherichia coli by metal chelating chromatography |
1.14.13.24 | single step purification using substrate-mediated interaction of the enzyme with blue-Sepharose, homogeneity |