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Search Purification (Commentary)

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Results 1 - 4 of 4
EC Number Purification (Commentary)
Show all pathways known for 1.3.8.12Display the reaction diagram Show all sequences 1.3.8.12-
Show all pathways known for 1.3.8.12Display the reaction diagram Show all sequences 1.3.8.12HisTrap column chromatography and Superdex 200 gel filtration
Show all pathways known for 1.3.8.12Display the reaction diagram Show all sequences 1.3.8.12recombinant His-tagged wild-tpye and mutant enzymes from Escherichia coli strain Rosetta pLys (DE3) by nickel affinity chromatography of cell-free supernatant, desalting gel filtration, tag cleavage by thrombin, gel filtration, and ultrafiltration
Show all pathways known for 1.3.8.12Display the reaction diagram Show all sequences 1.3.8.12recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) pLysS by nickel affinity chromatography and ultrafiltration
Results 1 - 4 of 4