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EC Number	Purification (Commentary)
1.4.1.27	isolation from mitochondria by ammunium sulfate fractionation and gel filtration
1.4.1.27	isolation of component T-protein
1.4.1.27	isolation of P-protein
1.4.1.27	purification of P-protein, and for T-protein. The procedure leads to the isolation of a nearly homogeneous form of T-protein but P-protein still is heterogeneous
1.4.1.27	the purified recombinant apo-H-protein is lipoylated and consequently activated in vitro with lipoyl-AMP as a lipoyl donor by lipoyltransferase

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Release 2023.1 (January 2023)