EC Number |
---|
1.5.99.B2 | - |
1.5.99.B2 | DE52 anion exchange column chromatography |
1.5.99.B2 | DEAE Toyopearl 650M column chromatography and Superdex 200 gel filtration |
1.5.99.B2 | HisTrap column chromatography and Hitrap Q column chromatography |
1.5.99.B2 | Ni-chelating column chromatography |
1.5.99.B2 | Ni-NTA column chromatography and Sephadex G-25 gel filtration |
1.5.99.B2 | Ni2+ affinity column chromatography |
1.5.99.B2 | Ni2+ NTA affinity column chromatography |
1.5.99.B2 | Ni2+-charged chelating Sepharose column chromatography and Sephacryl S-300 gel filtration |
1.5.99.B2 | recombinant C-terminally His6-tagged wild-type and mutant enzymes from Mycobacterium smegmatis mc24517 cells by nickel affinity chromatography |