EC Number   |
|---|
  1.7.1.17 | - |
| 1.7.1.17 | native enzyme 4500fold by anion exchange chromatography, Blue Sepharose affinity chromtography, gel filtration, hydroxyapatite chromatography, again anion exchange chromatography and hydrophobic interaction chromatography. Recombinant enzyme AcpD from Escherichia coli, strain JM109 by anion exchange chromatography, Blue Sepharose affinity chromtography, gel filtration, and chromatography on GIGAPITE resin, the purified gene product exhibits activity corresponding to that of the native azoreductase |
| 1.7.1.17 | Ni-NTA column chromatography |
| 1.7.1.17 | Ni-Sepharose column chromatography |
| 1.7.1.17 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 1.7.1.17 | recombinant His-tagged enzyme from Escherichia coli strain XL1-Blue by nickel affinity chromatography |
| 1.7.1.17 | recombinant His-tagged wild-type and mutant AzoR enzymes from Escherichia coli BL21(DE3) by nickel affinity chromatography |
| 1.7.1.17 | recombinant His-tagged wild-type and mutant enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography, dialysis, and ultrafiltration |
