EC Number   |
|---|
    1.7.2.4 | - |
| 1.7.2.4 | a pink form is isolated when the purification is done aerobically, a purple form when the purification is under anaerobic conditions |
| 1.7.2.4 | ammonium sulfate, DEAE-Sephadex A-50, Sephacryl S-200 |
| 1.7.2.4 | anaerobical purification of N2OR under an Ar atmosphere by three different steps of anion exchange chromatography, followed by cytochrome c affinity chromatography and ultrafiltration |
| 1.7.2.4 | DEAE cellulose column chromatography, hydroxyapatite column chromatography, Mono Q10 anion-exchange column chromatography, and Sephadex S-200 gel filtration |
| 1.7.2.4 | DEAE-FF ion exchange chromatography and Resource Q column chromatography |
| 1.7.2.4 | HiTrap Q Sepharose anion exchange chromatography |
| 1.7.2.4 | isolation of the enzyme in the purple form, in which the copper centre is in the oxidized [2Cu2+:2Cu+] redox state and is redox active. Isolation of this form in the presence of oxygen from a microaerobic culture in the presence of nitrate and also from a strictly anaerobic culture |
| 1.7.2.4 | native enzyme is a three-step procedure |
| 1.7.2.4 | of anaerobically grown cells |
