EC Number   |
|---|
   2.1.1.63 | - |
| 2.1.1.63 | amylose resin column chromatography, Toyopearl-SuperQ column chromatography, ammonium sulfate precipitation, and Toyopearl-ether 650M column chromatography |
| 2.1.1.63 | by immobilized metal affinity chromatography |
| 2.1.1.63 | DEAE-Sepharose column chromatography |
| 2.1.1.63 | expressed at high levels in Escherichia coli fused to an N-terminal polyhistidine tag that allows a single-step isolation and purification by metal-affinity chromatography |
| 2.1.1.63 | heterologous expression by His-tag chromatography affinity |
| 2.1.1.63 | HiTrapQ column chromatography, MonoQ column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration |
| 2.1.1.63 | immobilized metal affinity column chromatography |
| 2.1.1.63 | mutant V139F |
| 2.1.1.63 | mutants purified by either nickel or glutathione affinity chromatography |
