EC Number   |
|---|
    2.3.1.47 | - |
| 2.3.1.47 | from Escherichia coli DH5alpha, ammonium sulfate fractionation, gel filtration, hydrophobic interaction chromatography, ion exchange chromatography |
| 2.3.1.47 | Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography |
| 2.3.1.47 | Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, gel filtration |
| 2.3.1.47 | Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, heat treatment with centrifugation |
| 2.3.1.47 | Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, ion exchange chromatography |
| 2.3.1.47 | immobilized metal ion affinity chromatography (Ni2+), His-tag cleavage, immobilized metal ion affinity chromatography (Ni2+), gel filtration |
| 2.3.1.47 | maltose binding protein affinity chromatography |
| 2.3.1.47 | native enzyme partially by subcellular fractionation, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration |
| 2.3.1.47 | Ni-NTA column chromatography |
