EC Number   |
|---|
    2.4.2.17 | - |
| 2.4.2.17 | ammonium sulfate precipitation and DEAE-cellulose chromatography |
| 2.4.2.17 | centrifugation and DEAE-Sephacel anion-exchange column |
| 2.4.2.17 | chromatography on Sephadex G-75 |
| 2.4.2.17 | first purification by heating the crude extract, ammonium sulfate precipitation and selective histidine-dependent ammonium sulfate precipitation |
| 2.4.2.17 | HisTrap column chromatography |
| 2.4.2.17 | HisTrap resin column chromatography and Superdex S200 gel filtration |
| 2.4.2.17 | HiTrap Talon column chromatography and Superdex 200 gel filtration |
| 2.4.2.17 | HiTrap Talon resin column chromatography, GST-affinity column chromatography and Superdex S200 gel filtration |
| 2.4.2.17 | immobilized metal ion affinity chromatography (Ni2+) |
