EC Number   |
|---|
   3.1.1.53 | - |
| 3.1.1.53 | 180fold enrichment |
| 3.1.1.53 | 2600fold enrichment |
| 3.1.1.53 | 3000fold |
| 3.1.1.53 | 850fold enrichment |
| 3.1.1.53 | copurified with a 66000 Da esterase |
| 3.1.1.53 | from baculovirus infected insect Sf9 cells as GFP-fusion protein by ultracentrifugation, ion-exchange chromatography on Q-Sepharose FF column and dialysis |
| 3.1.1.53 | from fresh tissue by ultracentrifugation (dialysis of supernatant), followed by gel-filtration on Sephadex G-200 column, ion-exchange chromatography on Sephadex-DEAE-A-50 column, second ion-exchange chromatography on DEAE-Sephacel column, isoelectric focussing on ampholine of pH 3.5-9.5 and a saccharose gradient, and final dialysis, 866% purification compared to homogenate |
| 3.1.1.53 | from HEK293S cells by protein A-affinity chromatography followed by on-bead thrombin digestion |
| 3.1.1.53 | GSTrap column chromatography, and Superdex 200 gel filtration |
