EC Number   |
|---|
   4.2.1.119 | - |
| 4.2.1.119 | 0.2% yield,the microsomal isoform elutes at 0.4-0.7 MKCl, at low salt concentrations, whereas the peroxisomal isoform elutes at high salt concentrations |
| 4.2.1.119 | recombinant His-tagged MFE-2 from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography, removal of the His tag |
| 4.2.1.119 | recombinant His6-tagged PhaJ4 homologues from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 4.2.1.119 | recombinant protein purified from the cell extract to apparent homogeneity by three chromatographic steps on anion-exchange, cation-exchange and size-exclusion columns |
| 4.2.1.119 | recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) |
| 4.2.1.119 | recombinant wild-type and mutant GFP-tagged hydratase domains of multifunctional enzyme type 2 hydratase from Escherichia coli strain JM109 by anion exchange chromatography |
| 4.2.1.119 | the FOX2 gene is overexpressed from a multicopy vector (YEp352) in Saccharomyces cerevisiae and the gene product purified to apparent homogenity. A truncated version of MFP lacking 271 carboxyl-terminal amino acids is also overexpressed and purified |
