EC Number |
---|
4.4.1.20 | - |
4.4.1.20 | affinity chromatography purification based on specific interaction between leukotriene C4 synthase and microsomal glutathione S-transferase which ocurs in the presence of magnesium ion |
4.4.1.20 | extraction with a Triton X-100/Triton-DOC mixture, two affinity chromatography steps and gel filtration |
4.4.1.20 | fractioning |
4.4.1.20 | Ni-Sepharose column chromatography, S-hexylglutathione agarose chromatography, and Superdex 200 gel filtration |
4.4.1.20 | partial |
4.4.1.20 | solubilized with sodium deoxycholic acid and DDM, four steps of column chromatography, including affinity chromatography with S-hexyl GSH |
4.4.1.20 | the recombinant enzyme from Schizosaccharomyces pombe, to apparent homogeneity |
4.4.1.20 | to homogeneity |