EC Number   |
|---|
    5.4.2.8 | - |
| 5.4.2.8 | ammonium sulfate precipitation, ion exchange column chromatography, and Sephacryl S-100 gel filtration |
| 5.4.2.8 | ammonium sulfate, Sephadex G-25, KM-cellulose, partially purified |
| 5.4.2.8 | cell extracts are purified by His-tag agarose beads |
| 5.4.2.8 | DEAE-Sepharose column chromatography and Superdex 75 gel filtration |
| 5.4.2.8 | heat treatment 85°C, 20 min, anion-exchange, hydrophobic and gel filtration column chromatography |
| 5.4.2.8 | Ni-NTA column chromatography |
| 5.4.2.8 | Ni-Sepharose column chromatography |
| 5.4.2.8 | nickel affinity chromatography column chromatography |
| 5.4.2.8 | nickel-affinity column chromatography |
