EC Number |
Reaction |
Reference |
---|
1.1.1.159 | cholate + NAD+ = 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oate + NADH + H+ |
belongs to the short-chain dehydrogenase/reductase 1 familiy (SDR), mechanism |
286081 |
1.1.1.159 | cholate + NAD+ = 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oate + NADH + H+ |
catalyses the oxidation of the 7alpha-hydroxyl group of bile acids and alcohols both in their free and conjugated forms. The Bacteroides fragilis and Clostridium enzymes can also utilize NADP+ |
- |
1.1.1.159 | cholate + NAD+ = 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oate + NADH + H+ |
short-chain dehydrogenase group |
286084 |
1.1.1.159 | cholate + NAD+ = 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oate + NADH + H+ |
Tyr159 acts as basic catalyst, Lys163 binds to NAD(H) and lowers the pKa value of Tyr159, Ser146 stabilizes the substrate, reaction intermediate and product in catalysis |
286083 |