EC Number |
Reaction |
Reference |
---|
1.3.5.2 | (S)-dihydroorotate + a quinone = orotate + a quinol |
- |
- |
1.3.5.2 | (S)-dihydroorotate + a quinone = orotate + a quinol |
68% identity with type A enzyme of Lactococcus lactis, two-site non-classical ping-pong kinetic mechanism |
390930 |
1.3.5.2 | (S)-dihydroorotate + a quinone = orotate + a quinol |
different binding sites for dihydroorotate and the electron acceptor, two-site ping-pong mechanism. Cleavage site at R182 is conserved between the two major families of dihydroorotate dehydrogenases, it is positioned in a loop, which is crucial for catalysis but irrelevant for protein stability |
390918 |
1.3.5.2 | (S)-dihydroorotate + a quinone = orotate + a quinol |
enzyme uses a stepwise mechanism for dihydroorotate oxidation |
696348 |
1.3.5.2 | (S)-dihydroorotate + a quinone = orotate + a quinol |
existence of 3 families differing in their selectivity for oxidizing substrates: 1A are soluble, containing FMN and use fumarate, 1B are soluble, one subunit contains an iron-sulfur center, FAD and reduces NAD+, family 2 enzymes are membrane-bound, contain FMN and are oxidized by ubiquinone. Mechanism consists of 3 reaction phases. Different binding-mechanisms for enzyme the reaction-steps are suggested |
390919 |
1.3.5.2 | (S)-dihydroorotate + a quinone = orotate + a quinol |
fourth step in biosynthesis of pyrimidines. Two domains: alpha/beta-barrel domain containing the active site, one alpha-helical domain that forms the opening of a tunnel leading to active site |
390920 |
1.3.5.2 | (S)-dihydroorotate + a quinone = orotate + a quinol |
in liver, myocardium and skeletal muscle tissues the activity intensities vary from animal to animal, but are similar in ileum, colon and kidney cortex. Cardiac enzyme expresses a pronounced oxidase activity |
390925 |
1.3.5.2 | (S)-dihydroorotate + a quinone = orotate + a quinol |
one-site ping-pong mechanism, residues 129-137 form a flexible loop, responsible for substrate binding |
390918 |
1.3.5.2 | (S)-dihydroorotate + a quinone = orotate + a quinol |
ping-pong mechanism |
390918, 391223, 391226 |
1.3.5.2 | (S)-dihydroorotate + a quinone = orotate + a quinol |
soluble quinones as second substrate |
391223 |