1.4.1.23 | L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+ |
the reductive amination proceeds through a sequential ordered ternary-binary mechanism. NADH binds to the enzyme first, followed by 2-oxoisovalerate and NH3, and valine is released first, followed by NAD+ |
391694 |