    1.8.98.5 | 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+ = 2 H2 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB |
the two HdrA subunits form the interprotomer contact which implicates an electronic connection between the two FADs and two [4Fe-4S] clusters. The bifurcating FAD buried inside HdrA is the core of the complex from which three electron routes branch off. The single electrons from the [NiFe] center flow to the FAD from which a high-potential electron is transferred to the non-cubane (nc) [4Fe-4S] clusters and a low-potential electron to the Fd domain. FAD-binding site in HdrA with a isoalloxazine ring that is localized between two Rossmann fold domains, the two linkers between them and the adjacent HdrA partner. The most striking interaction is formed between N5 and the positively charged Lys409 that is kept at its position by interactions with Glu356, Lys187-O and a H2O multiply linked with the polypeptide. The electron transfer route is interrupted between the [2Fe-2S] cluster of MvhD and FAD |
-, 764200 |
