evolution
nitroreductases are a family of flavoproteins that catalyze the NAD(P)H-dependent reduction of nitro groups to hydroxyamino and/or amino groups on vari-ous nitro-substituted compounds
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the enzyme crystal structure of NfsB, PDB ID 1DS7, is used for molecular ligand docking studies
physiological function
Escherichia coli NfsB expressing HCT-116 cells are 23.5fold more sensitive to prodrug nitro-CBI-DEI than pure cultures of untransfected cells, monolayer cytotoxicity assays using human colon carcinoma HCT-116 cells. The discrepancy in the fold-sensitivity to nitro-CBI-DEI between the two and three dimensional cytotoxicity assays stems from loss of activated drug into the media in the monolayer cultures
physiological function
Pseudomonas aeruginosa NfsB expressing HCT-116 cells are 540fold more sensitive to prodrug nitro-CBI-DEI than pure cultures of untransfected cells, the activated drug that they generate also displays an unprecedented local bystander effect, monolayer cytotoxicity assays using human colon carcinoma HCT-116 cells. The discrepancy in the fold-sensitivity to nitro-CBI-DEI between the two and three dimensional cytotoxicity assays stems from loss of activated drug into the media in the monolayer cultures
