EC Number   |
General Information |
Reference |
|---|
   1.14.13.215 | evolution |
enzyme AsuE1 belongs to the family of single-component monooxygenases: a single enzyme catalyzes both the reduction of flavin by NADPH, and the hydroxylation using molecular oxygen and reduced flavin cofactor |
-, 735926 |
| 1.14.13.215 | malfunction |
disruption of asuE2 significantly decreases the yield of asukamycin and results in the accumulation of protoasukamycin |
-, 735926 |
| 1.14.13.215 | metabolism |
AsuE1 and AsuE3, both single-component flavin-dependent monooxygenases, catalyze the exquisite regio- and enantiospecific postpolyketide synthase (PKS) assembly oxygenations. AsuE1 installs a hydroxyl group on the 3,4-AHB ring to form a 4-hydroxyquinone moiety, which is epoxidized by AsuE3 to yield the epoxyquinone functionality. Despite being a single-component monooxygenase, AsuE1 activity is elicited by AsuE2, a pathway-specific flavin reductase |
-, 735926 |
| 1.14.13.215 | metabolism |
in the asukamycin metabolism, enzymes AsuE1 and AsuE2 catalyze the conversion of protoasukamycin to 4-hydroxyprotoasukamycin, which is epoxidized at C5-C6 by enzyme AsuE3 to the final product, asukamycin. Synthesis and structures of asukamycin and related metabolites, overview |
736406 |
