  1.3.1.107 | physiological function |
RNAi-based silencing of sanguinarine reductase leads to displayed growth rates and morphology comparable to the wild type, but strains show either less or more of the slightly red color typical of benzophenanthridine producing cultures. Silenced strains give rise to mutants that either show a complete stop of elicitor-triggered alkaloid production or a burst of biosynthesis that severalfold surpasses the wild type level. The enzyme substrate, sanguinarine, inhibits phospholipase A2 at the plasma membrane, an initial component of the signal path towards expression of biosynthetic enzymes. The product, dihydrosanguinarine, inhibits enzymes of early biosynthesis, prior to reticuline formation. By tuning these steady states, sanguinarine reductase adjusts the capacity of alkaloid biosynthesis |
730597 |
