EC Number |
General Information |
Reference |
---|
3.5.99.2 | malfunction |
thi20 deletion mutant cells can not grow in the presence of the synthetic thiamine antagonists, pyrithiamine and oxythiamin, indicating that the THI20 gene is necessary for the utilization of pyrithiamine and oxythiamine as precursors for thiamin |
686824 |
3.5.99.2 | metabolism |
TenA plays a pivotal role in the thiamin biosynthetic route |
711977 |
3.5.99.2 | metabolism |
thiamin-degradation products are hydrolyzed by thiaminase II, yielding 4-amino-5-hydroxymethyl-2-methylpyrimidine. This compound is an intermediate in thiamin biosynthesis. The enzyme is involved in the biosynthesis of thiamin diphosphate, the active form of the cofactor |
718477 |
3.5.99.2 | metabolism |
thiaminase is involved in vitamin B1 metabolism |
713350 |
3.5.99.2 | metabolism |
thiaminase type II, TenA, catalyzes the deamination of aminopyrimidines, including the cleavage of thiamine to 4-amino-5-hydroxymethyl-2-methylpyrimidine and 5-(2-hydroxyethyl)-4-methylthiazole in the metabolism of thiamine (vitamin B1), in Staphylococcus aureus |
718509 |
3.5.99.2 | more |
putative active-site residues are Asp44, Cys137, Tyr167 and Glu208 |
718509 |
3.5.99.2 | more |
THI20 is a trifunctional enzyme containing an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain and a C-terminal thiaminase II (TenA-like) domain |
718477 |
3.5.99.2 | physiological function |
thiaminase II activity of Thi20p is involved in the thiamine salvage pathway |
686824 |