EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.1.99.20 | 0.24 |
- |
PEG-1000 |
mutant enzyme H465A, in 0.1 M Tris-HCl buffer (pH 8.0), at 25°C |
725161 |
1.1.99.20 | 0.54 |
- |
PEG-mono-4-nonylphenyl ether, averaging 10 ethoxy units |
mutant enzyme H465A, in 0.1 M Tris-HCl buffer (pH 8.0), at 25°C |
725161 |
1.1.99.20 | 1.3 |
- |
PEG-mono-4-nonylphenyl ether, averaging 10 ethoxy units |
mutant enzyme N90A, in 0.1 M Tris-HCl buffer (pH 8.0), at 25°C |
725161 |
1.1.99.20 | 1.9 |
- |
PEG-1000 |
mutant enzyme N90A, in 0.1 M Tris-HCl buffer (pH 8.0), at 25°C |
725161 |
1.1.99.20 | 3.6 |
- |
PEG-1000 |
wild type enzyme, in 0.1 M Tris-HCl buffer (pH 8.0), at 25°C |
725161 |
1.1.99.20 | 5.2 |
- |
PEG-mono-4-nonylphenyl ether, averaging 10 ethoxy units |
wild type enzyme, in 0.1 M Tris-HCl buffer (pH 8.0), at 25°C |
725161 |