EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
3.5.1.2 | 0.19 |
- |
L-glutamine |
mutant Q63E, cooperative kinetic toward L-Gln, Hill coefficient 2.3, pH 7.5, 37°C |
755075 |
3.5.1.2 | 0.47 |
- |
L-glutamine |
wild-type, cooperative kinetic toward L-Gln, Hill coefficient 2.0, pH 7.5, 37°C |
755075 |
3.5.1.2 | 0.63 |
- |
L-glutamine |
pH 8.5, temperature not specified in the publication, isozyme GLS2 |
718889 |
3.5.1.2 | 2.8 |
- |
Glutaramate |
pH 7.2, 30°C |
711316 |
3.5.1.2 | 5.3 |
- |
L-glutamine |
pH 8.5, temperature not specified in the publication, isozyme GLS1 |
718889 |
3.5.1.2 | 6.9 |
- |
Glutaramate |
pH 8.5, 30°C |
711316 |
3.5.1.2 | 8.7 |
- |
succinamate |
pH 7.2, 30°C |
711316 |
3.5.1.2 | 16 |
- |
L-glutamine |
pH 8.5, temperature not specified in the publication, wild-type GAC and GAC mutant F322S/F318Y |
718889 |
3.5.1.2 | 20 |
- |
succinamate |
pH 8.5, 30°C |
711316 |
3.5.1.2 | 29.6 |
- |
L-asparagine |
mutant T169M, cooperative kinetic toward L-Asn, Hill coefficient 1.8, pH 7.5, 37°C |
755075 |