EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
4.3.2.10 | -999 |
- |
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide |
pH 7.0, 25°C, kinetic constants are determined for wild-type and mutant enzymes |
747285 |
4.3.2.10 | -999 |
- |
L-glutamine |
pH 7.0, 25°C, kinetic constants are determined for wild-type and mutant enzymes |
747285 |
4.3.2.10 | 0.00042 |
- |
L-glutamine |
pH 8.0, 37°C, HisF mutant enzyme V12A |
749186 |
4.3.2.10 | 0.00046 |
- |
L-glutamine |
pH 8.0, 37°C, wild-type enzyme |
749186 |
4.3.2.10 | 0.000758 |
- |
L-glutamine |
pH 8.0, 30°C |
747861 |
4.3.2.10 | 0.00087 |
- |
L-glutamine |
pH 8.0, 37°C, HisF mutant enzyme V48A |
749186 |
4.3.2.10 | 0.00107 |
- |
L-glutamine |
pH 8.0, 37°C, HisF mutant enzyme D98A |
749186 |
4.3.2.10 | 0.0018 |
- |
L-glutamine |
pH 8.0, 37°C, HisF mutant enzyme K19A |
749186 |
4.3.2.10 | 0.0121 |
- |
L-glutamine |
pH 8.0, 40°C |
747861 |
4.3.2.10 | 0.024 |
- |
NH4+ |
pH 7.0, 30°C |
749228 |