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EC Number Renatured (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.76denaturing of the enzyme by Triton buffer, refolding of the enzyme from inclusion bodies after recombinant expression in Escherichia coli in Tris-HCl, pH 8.0, containing GSSG and GSH at 25°C 664410
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.76recombinant His-tagged enzyme from Escherichia coli strain M15 is solubilized in 8 M urea, immobilized on Ni2+-nitrilotriacetate-agarose matrix, and refolded by gradual removal of the chaotropic agent 715799
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