EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
1.13.11.27 | -999 |
- |
activity of 0.0033 micromol/min/mg for 2YT-grown Escherichia coli DH5alpha extract and 0.0018 micromol/min/mg for LB-grown Escherichia coli DH5alpha |
677278 |
1.13.11.27 | -999 |
- |
methods for assay |
395389 |
1.13.11.27 | -999 |
- |
the oxygen electrode provides an excellent measure of enzyme activity under conditions where maximum enzyme activity is obtainable in the absence of a reducing agent |
395374 |
1.13.11.27 | -999 |
- |
with substrate 2,3,5,6-tetraproteo 4-hydroxyphenylpyruvate and the solvent H2O Vmax = 1.9/s, with substrate 2,3,5,6-tetradeutero 4-hydroxyphenylpyruvate and the solvent H2O Vmax = 1.8/s, with substrate 2,3,5,6-tetraproteo 4-hydroxyphenylpyruvate and the solvent D2O Vmax = 0.86/s |
672033 |
1.13.11.27 | 0.004 |
- |
protein expressed in Escherichia coli DH5alpha |
677278 |
1.13.11.27 | 0.04 |
- |
purified recombinant mutant R378K, pH 6.5, 37°C, homogentisate production |
726347 |
1.13.11.27 | 0.06 |
- |
purified recombinant mutant E254D, pH 6.5, 37°C, O2 consumption |
726347 |
1.13.11.27 | 0.07 |
- |
purified recombinant mutant R378K, pH 6.5, 37°C, O2 consumption |
726347 |
1.13.11.27 | 0.11 |
0.2 |
protein expressed in Escherichia coli SG13009 |
677278 |
1.13.11.27 | 0.17 |
0.26 |
protein expressed in Escherichia coli M15(pRep4) |
677278 |