(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+
-
2-oxoadipate + CO2 + NADH + H+
-
?
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+
-
2-oxoadipate + CO2 + NADH + H+
-
?
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+
strict specificity for homoisocitrate
2-oxoadipate + CO2 + NADH + H+
-
?
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+
i.e. (2R,3S)-homoisocitrate
2-oxoadipate + CO2 + NADH + H+
-
?
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+
strict specificity for homoisocitrate, the enzyme selectively binds the Mg(II):homoisocitrate complex
2-oxoadipate + CO2 + NADH + H+
-
?
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+
-
2-oxoadipate + CO2 + NADH + H+
-
?
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+
strict specificity for homoisocitrate
2-oxoadipate + CO2 + NADH + H+
-
?
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+
strict specificity for homoisocitrate, the enzyme selectively binds the Mg(II):homoisocitrate complex
2-oxoadipate + CO2 + NADH + H+
-
?
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+
-
2-oxoadipate + NADH + H+ + CO2
-
?
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+
homoisocitrate dehydrogenase catalyzes the Mg2+- and K+-dependent oxidative decarboxylation of homoisocitrate to alpha-ketoadipate using NAD as the oxidant, it utilizes a Lys-Tyr pair to catalyze the acid-base chemistry of the reaction, the active site Lys-Tyr pair consists of lysine 206 and tyrosine 150
2-oxoadipate + NADH + H+ + CO2
-
?
