EC Number   |
Substrates |
Organism  |
Products |
Reversibility |
|---|
    1.3.1.85 | (E)-crotonyl-CoA + CO2 + NADPH + H+ |
100% relative specific activity, the carboxylation reaction is the physiologically relevant reaction |
Cereibacter sphaeroides |
(2S)-ethylmalonyl-CoA + NADP+ |
- |
r |
| 1.3.1.85 | (E)-crotonyl-CoA + NADPH + H+ |
10% of maximal activity (compared with vmax of crotonyl-CoA carboxylation) |
Cereibacter sphaeroides |
butyryl-CoA + NADP+ |
- |
? |
| 1.3.1.85 | acryloyl-CoA + CO2 + NADPH + H+ |
acryloyl-CoA is accepted as an alternative substrate analogue by the enzyme with 40% relative activity (compared with vmax of crotonyl-CoA carboxylation) |
Cereibacter sphaeroides |
methylmalonyl-CoA + NADP+ |
- |
? |
| 1.3.1.85 | crotonyl-CoA + CO2 + NADPH + H+ |
- |
Cereibacter sphaeroides |
(2S)-ethylmalonyl-CoA + NADP+ |
- |
r |
| 1.3.1.85 | more |
does not reduce crotonyl-N-acetylcysteamine, methacryloyl-CoA, 6-hydroxycylohex-1-ene-1-carboxyl-CoA, cyclo-hexa-1,5-diene-1-carboxyl-CoA, acetoacetyl-CoA, propionyl-CoA, (R)-3-hydroxybutyryl-CoA, (S)-3-hydroxybutyryl-CoA (less than 1% specific activity) |
Cereibacter sphaeroides |
? |
- |
? |
| 1.3.1.85 | more |
the enzyme also catalyzes the reduction of crotonyl-CoA to butyryl-CoA in the absence of CO2, but with only about one tenth of the maximum rate |
Cereibacter sphaeroides |
? |
- |
? |
| 1.3.1.85 | crotonyl-CoA + CO2 + NADPH + H+ |
crotonyl-CoA is the best substrate |
Cereibacter sphaeroides 2.4.1 |
(2S)-ethylmalonyl-CoA + NADP+ |
- |
r |
| 1.3.1.85 | crotonyl-CoA + NADPH + H+ |
reduction at low rate |
Cereibacter sphaeroides 2.4.1 |
butyryl-CoA + NADP+ |
- |
ir |
| 1.3.1.85 | crotonyl-CoA + CO2 + NADPH + H+ |
- |
Kitasatospora setae |
(2S)-ethylmalonyl-CoA + NADP+ |
- |
? |
| 1.3.1.85 | more |
the enzyme catalyzes the reduction of crotonyl-CoA to butyryl-CoA as a side reaction, but only in the absence of CO2 and at low catalytic efficiency |
Kitasatospora setae |
? |
- |
- |
