EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.1.1.358 | 1-methylisatin + NADPH + H+ |
86% of the activity compared to isatin |
Candida parapsilosis |
? |
- |
? |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ |
- |
Candida parapsilosis |
(R)-pantolactone + NADP+ |
- |
ir |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ |
- |
Candida parapsilosis |
(R)-pantolactone + NADP+ |
- |
? |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ |
- |
Candida dubliniensis |
(R)-pantolactone + NADP+ |
- |
? |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ |
- |
Candida orthopsilosis |
(R)-pantolactone + NADP+ |
- |
? |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ |
69% of the activity compared to isatin. The stereospecificity for NADPH is not known. Activity with NADH is 2% compared to the activity with NADPH |
Candida parapsilosis |
(R)-pantolactone + NADP+ |
- |
ir |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ |
recombinant CorCPR exhibited a high catalytic activity for the synthesis of (R)-pantolactone ((R)-PL) from 2-dehydropantolactone (KPL). The activity assay is performed using purified CorCPR and crude recombinant GDH in a single batch reaction. GDH is used to complete the enzyme-coupled NADPH regeneration system. CPR-01 form Candida orthopsilosis Co 90-125 exhibits excellent enantioselectivity (enantiomeric excess of over 99%). CorCPR exhibits the highest activity and stereoselectivity toward KPL |
Candida orthopsilosis |
(R)-pantolactone + NADP+ |
- |
? |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ |
- |
Candida dubliniensis ATCC MYA-646 |
(R)-pantolactone + NADP+ |
- |
? |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ |
- |
Candida dubliniensis CD36 |
(R)-pantolactone + NADP+ |
- |
? |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ |
- |
Candida orthopsilosis Co 90-125 |
(R)-pantolactone + NADP+ |
- |
? |