EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.13.128 | 1,3,7-trimethylxanthine + O2 + NAD(P)H + H+ |
i.e. caffeine |
Pseudomonas putida |
3,7-dimethylxanthine + formaldehyde + NAD(P)+ |
i.e. theobromine |
? |
1.14.13.128 | 1,3,7-trimethylxanthine + O2 + NADPH + H+ |
i.e. caffeine |
Pseudomonas putida |
1,3-dimethylxanthine + formaldehyde + NADP+ + H2O |
i.e. theophylline |
? |
1.14.13.128 | 1,3,7-trimethylxanthine + O2 + NADPH + H+ |
i.e. caffeine |
Pseudomonas putida |
1,7-dimethylxanthine + formaldehyde + NADP+ + H2O |
i.e. paraxanthine |
? |
1.14.13.128 | 1,7-dimethylxanthine + 2 O2 + 2 NADH + 2 H+ |
i.e. paraxanthine, NADH is the preferred cofactor of reductase component of the N-demethylase holoenzyme (Ccr). The product 7-methylxanthine is further demethylated to xanthine. 1,7-Dimethylxanthine (paraxanthine) demethylation is 22% of the activity compared to demethylation of 7-methylxanthine. Activity is absolutely dependent of oxygen as a cosubstrate |
Pseudomonas putida |
xanthine + 2 NAD+ + 2 H2O + 2 formaldehyde |
- |
? |
1.14.13.128 | 1,7-dimethylxanthine + 2 O2 + 2 NADH + 2 H+ |
i.e. paraxanthine, NADH is the preferred cofactor of reductase component of the N-demethylase holoenzyme (Ccr). The product 7-methylxanthine is further demethylated to xanthine. 1,7-Dimethylxanthine (paraxanthine) demethylation is 22% of the activity compared to demethylation of 7-methylxanthine. Activity is absolutely dependent of oxygen as a cosubstrate |
Pseudomonas putida CBB5 |
xanthine + 2 NAD+ + 2 H2O + 2 formaldehyde |
- |
? |
1.14.13.128 | 1,7-dimethylxanthine + 2 O2 + 2 NADPH + 2 H+ |
i.e. paraxanthine, activity of the reductase component of the N-demethylase holoenzyme (Ccr) with NADPH is 22% of that with NADH. The enzyme also catalyzes the further demethylation of the product 7-methylxanthine to xanthine. Activity is absolutely dependent of oxygen as a cosubstrate |
Pseudomonas putida |
xanthine + 2 NADP+ + 2 H2O + 2 formaldehyde |
- |
? |
1.14.13.128 | 1,7-dimethylxanthine + 2 O2 + 2 NADPH + 2 H+ |
i.e. paraxanthine, activity of the reductase component of the N-demethylase holoenzyme (Ccr) with NADPH is 22% of that with NADH. The enzyme also catalyzes the further demethylation of the product 7-methylxanthine to xanthine. Activity is absolutely dependent of oxygen as a cosubstrate |
Pseudomonas putida CBB5 |
xanthine + 2 NADP+ + 2 H2O + 2 formaldehyde |
- |
? |
1.14.13.128 | 1,7-dimethylxanthine + O2 + NADH + H+ |
i.e. paraxanthine, NADH is the preferred cofactor of reductase component of the N-demethylase holoenzyme (Ccr). The product 7-methylxanthine is further demethylated to xanthine. 1,7-Dimethylxanthine (paraxanthine) demethylation is 22% of the activity compared to demethylation of 7-methylxanthine. Activity is absolutely dependent of oxygen as a cosubstrate |
Pseudomonas putida |
7-methylxanthine + NAD+ + H2O + formaldehyde |
- |
? |
1.14.13.128 | 1,7-dimethylxanthine + O2 + NADH + H+ |
i.e. paraxanthine, NADH is the preferred cofactor of reductase component of the N-demethylase holoenzyme (Ccr). The product 7-methylxanthine is further demethylated to xanthine. 1,7-Dimethylxanthine (paraxanthine) demethylation is 22% of the activity compared to demethylation of 7-methylxanthine. Activity is absolutely dependent of oxygen as a cosubstrate |
Pseudomonas putida CBB5 |
7-methylxanthine + NAD+ + H2O + formaldehyde |
- |
? |
1.14.13.128 | 1,7-dimethylxanthine + O2 + NADPH + H+ |
i.e. paraxanthine, activity of the reductase component of the N-demethylase holoenzyme (Ccr) with NADPH is 22% of that with NADH. The enzyme also catalyzes the further demethylation of the product 7-methylxanthine to xanthine. Activity is absolutely dependent of oxygen as a cosubstrate |
Pseudomonas putida |
7-methylxanthine + NADP+ + H2O + formaldehyde |
- |
? |