EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.14.115 | 11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] |
single oxidation reaction catalyzed by CYP72A154 |
Glycyrrhiza uralensis |
30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.115 | 11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] |
the enzyme mainly catalyzes the C-30 hydroxylation of 11-oxo-beta-amyrin to yield 30-hydroxy-11-oxo-beta-amyrin as the major product, accompanied by its isomers as minor products |
Glycyrrhiza uralensis |
30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.115 | 30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] |
- |
Glycyrrhiza uralensis |
glycyrrhetaldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O |
- |
? |
1.14.14.115 | 30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] |
single oxidation reaction catalyzed by CYP72A154 |
Glycyrrhiza uralensis |
glycyrrhetaldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O |
- |
? |
1.14.14.115 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 |
hydroxylation at C-16beta, main activity of enzyme CYP716A141 |
Platycodon grandiflorus |
maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.115 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 |
hydroxylation at C-28, reaction of EC 1.14.13.201 |
Platycodon grandiflorus |
oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.115 | glycyrrhetaldehyde + O2 + [reduced NADPH-hemoprotein reductase] |
- |
Glycyrrhiza uralensis |
glycyrrhetinate + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.115 | maniladiol + [reduced NADPH-hemoprotein reductase] + O2 |
hydroxylation at C-28 |
Platycodon grandiflorus |
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.115 | more |
CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry |
Platycodon grandiflorus |
? |
- |
? |
1.14.14.115 | oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2 |
hydroxylation at C-16beta |
Platycodon grandiflorus |
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |