EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.14.149 | 1-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Nicotiana tabacum |
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.149 | 1beta-hydroxy-5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2 |
the catalytic efficiency for 1beta-hydroxy-5-epiaristolochene is about 10times greater than that for 3alpha-hydroxy-5-epiaristolochene |
Nicotiana tabacum |
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.149 | 3-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Nicotiana tabacum |
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.149 | 3alpha-hydroxy-5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2 |
the catalytic efficiency for 3alpha-hydroxy-5-epiaristolochene is about 10times lower than that for 1beta-hydroxy-5-epiaristolochene |
Nicotiana tabacum |
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.149 | 5-epiaristolochene + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 |
- |
Nicotiana tabacum |
capsidiol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O |
- |
? |
1.14.14.149 | 5-epiaristolochene + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 |
EAH mediates regio- and stereospecific hydroxylations at the C1 and C3 positions of 5-epi-aristolochene |
Nicotiana tabacum |
capsidiol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O |
- |
? |
1.14.14.149 | 5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Nicotiana tabacum |
3-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.149 | 5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2 |
3-deoxycapsidiol is a reaction product when EAH is incubated with high concentrations (above 0.4 mM) of the 5-epiaristolochene substrate |
Nicotiana tabacum |
3-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.149 | 5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Nicotiana tabacum |
1-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.149 | 5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2 |
the release of an 1beta-hydroxy-5-epiaristolochene intermediate at high 5-epiaristolochene concentrations and a 10fold catalytic preference for 1beta-hydroxy-5-epiaristolochene versus 3alpha-hydroxy-5-epiaristolochene is indicative of a preferred reaction order of hydroxylation at C-1, followed by that at C-3 |
Nicotiana tabacum |
1beta-hydroxy-5-epiaristolochene + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |