EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.14.151 | (+)-valencene + [reduced NADPH-hemoprotein reductase] + O2 |
enzyme HPO-mediated biohydroxylation of (+)-valencene |
Hyoscyamus muticus |
alpha-nootkatol + beta-nootkatol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.151 | (-)-vetispiradiene + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Hyoscyamus muticus |
solavetivol + [oxidized NADPH-hemoprotein reductase] + H2O |
solavetivol is also named premnaspirodien-2alpha-ol or (2S,4R,5S,7R)-spirovetiva-1(10),11(12)-dien-2-ol |
? |
1.14.14.151 | 4-epi-eremophilene + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Hyoscyamus muticus |
4-epieremophilen-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.151 | 5-epiaristolochene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 |
substrate affinity for 5-epiaristolochene is 2-4times lower than that for valencene or (-)-premnaspirodiene, respectively. HPO catalyzes the conversion of 5-epiaristolochene to four mono-hydroxylated products with 2beta-hydroxy-epiaristolochene comprising greater than 80% of the reaction products, 1beta-hydroxy-epiaristolochene about 5%, 3alpha-hydroxy-epiaristolochene less than 2%, and an unknown mono-hydroxylated product of about 20% |
Hyoscyamus muticus |
2beta-hydroxy-5-epiaristolochene + 1beta-hydroxy-5-epiaristolochene + 3alpha-hydroxy-5-epiaristolochene + 3 [oxidized NADPH-hemoprotein reductase] + 3 H2O |
- |
? |
1.14.14.151 | cedr-8-ene + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Hyoscyamus muticus |
cedr-8-en-15-ol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.151 | more |
HPO also catalyzes the equivalent regio-specific (C-2) hydroxylation of several eremophilane-type (decalin ring system) sesquiterpenes, such as with 5-epi-aristolochene |
Hyoscyamus muticus |
? |
- |
? |
1.14.14.151 | more |
the enzyme fails to convert nootkatol to nootkatone |
Hyoscyamus muticus |
? |
- |
? |
1.14.14.151 | more |
co-expression of enzyme HPO and cytochrome P450 reductase (CPR) leads to the hydroxylation of externally added (+)-valencene to trans-nootkatol in Pichia pastoris cells |
Hyoscyamus muticus |
? |
- |
? |
1.14.14.151 | valencene + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 |
- |
Hyoscyamus muticus |
alpha-nootkatol + beta-nootkatol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O |
- |
? |