EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
    1.14.15.1 | (+)-alpha-pinene + putidaredoxin + O2 |
- |
Pseudomonas putida |
(+)-cis-verbenol + (+)-myrtenol + (+)-verbenone + oxidized putidaredoxin + H2O |
- |
? |
| 1.14.15.1 | (+)-alpha-pinene + putidaredoxin + O2 |
- |
Pseudomonas putida |
? + oxidized putidaredoxin + H2O |
- |
? |
| 1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin |
- |
Pseudomonas putida |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O |
- |
? |
| 1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin |
terminal monooxygenase in a three-component camphor-hydroxylating system from Pseudomonas putida, the reaction cycle requires two distinct electron transfer processes from the [2Fe-2S] containing putidaredoxin to P450cam |
Pseudomonas putida |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O |
- |
? |
| 1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin |
analysis of protein-protein interactions between enzyme and cofactor, overview |
Pseudomonas putida |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O |
- |
? |
| 1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin |
binding of camphor is strongly dependent on the concentration of alcohols, alcohol expels camphor out of the heme cavity of the enzyme by affecting tertiary structure of Cyt P450cam as well as by modifying the solubility properties of camphor in aqueous medium, overview |
Pseudomonas putida |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O |
- |
? |
| 1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin |
determination of appearance of transient intermediates at 3°C by double mixing rapid scanning stopped-flow spectroscopy, electron transfer from reduced putidaredoxin gives high spin deoxyferrous P-450, substrate-free enzyme also binds the cofactor, but the cofactor does not deliver the electrons to the substrate-free oxyferrous enzyme, binding structure of camphor-bound oxyferrous P450-CAM with reduced putidaredoxin, functional implications of the formation of the perturbed oxyferrous intermediate, overview |
Pseudomonas putida |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O |
- |
? |
| 1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin |
mechanism of camphor hydroxylation incorporating an NADH-regeneration system, overview |
Pseudomonas putida |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O |
- |
? |
| 1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin |
regio- and stereoselective C-H bond hydroxylation, rebound mechanism, overview |
Pseudomonas putida |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O |
- |
? |
| 1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin |
regio- and stereospecific hydroxylation |
Pseudomonas putida |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O |
- |
? |
