EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   5.4.99.25 | more |
efficient formation of 5-fluoro-6-hydroxypseudouridine55 from 5-fluorouridine55 is catalyzed by wild-type enzyme and mutant enzymes Y67F and Y67L |
Thermotoga maritima |
? |
- |
? |
| 5.4.99.25 | more |
RNA containing 5-fluorouridine is a substrate |
Escherichia coli |
? |
- |
? |
| 5.4.99.25 | more |
the enzyme binds much more weakly to small RNA Pab91 than to small RNA Pab21. The Pab91 small ribonucleoprotein particle exhibits a higher catalytic efficiency than the Pab21 small ribonucleoprotein particle. Efficient aCBF5 binding probably relies on the pseudouridylation pocket which is not optimized for high activity in the case of Pab21 |
Pyrococcus abyssi |
? |
- |
? |
| 5.4.99.25 | tRNA uridine55 |
- |
Escherichia coli |
tRNA pseudouridine55 |
- |
? |
| 5.4.99.25 | tRNA uridine55 |
- |
Lupinus luteus |
tRNA pseudouridine55 |
- |
? |
| 5.4.99.25 | tRNA uridine55 |
- |
Homo sapiens |
tRNA pseudouridine55 |
- |
? |
| 5.4.99.25 | tRNA uridine55 |
- |
Thermotoga maritima |
tRNA pseudouridine55 |
- |
? |
| 5.4.99.25 | tRNA uridine55 |
- |
Methanocaldococcus jannaschii |
tRNA pseudouridine55 |
- |
? |
| 5.4.99.25 | tRNA uridine55 |
Pus4 catalyses the formation of pseudouridine55 in both mitochondrial and cytoplasmic tRNAs |
Saccharomyces cerevisiae |
tRNA pseudouridine55 |
- |
? |
| 5.4.99.25 | tRNA uridine55 |
aCbf5 and aGar1 together can function as a tRNA pseudouridine55 synthase in a guide RNA-independent manner. This activity is enhanced by aNop10, but not by L7Ae. The aCbf5 alone can also produce pseudouridine55 in tRNAs that contain the canonical 3-CCA sequence and this activity is stimulated by aGar1. The presence of C (or U) and A at tRNA position 56 and 58, respectively are not essential for Cbf5-mediated PSI55 formation. Variation in the structure of the anticodon arm of the tRNA does not affect the PSI55 synthase activity |
Methanocaldococcus jannaschii |
tRNA pseudouridine55 |
- |
? |
