EC Number |
Subunits |
Reference |
---|
1.13.12.19 | monomer |
1 * 39444, calculated, 1 * 42000, SDS-PAGE |
714009 |
1.13.12.19 | monomer |
1 * 40000, recombinant detagged enzyme, SDS-PAGE, 1 * 39670, recombinant detagged enzyme, mass spectrometry |
-, 741936 |
1.13.12.19 | monomer |
1 * 42000, SDS-PAGE |
715784 |
1.13.12.19 | monomer |
by SDS-PAGE and gel filtration |
743692 |
1.13.12.19 | More |
the overall PsEFE fold comprises 10 alpha-helices and 14 beta-strands, of which eight beta-strands (I-VIII) form the major and minor beta-sheets of the conserved distorted double-stranded beta helix (DSBH): the 2-oxoglutarate oxygenase characteristic fold. beta-Strands beta1 and beta2 at the N-terminus extend the major beta-sheet at the end of the DSBH away from the active site, beta-strands beta3 and beta6 extend the other end of the major beta-sheet close to the active site. alpha-Helices alpha2 and alpha5 bind across the surface of the major beta-sheet and likely stabilize it. A loop region (residues 80-93), located between beta3 and beta6, which harbors beta4 and beta5, acts as a lid partially covering the active site and provides residues that bind the L-Arg cofactor/substrate. Three alpha-helices (alpha8, alpha9, alpha10) at the C-terminus also contribute to the active site |
743692 |