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EC Number
Cloned (Commentary)
Reference
cloned from the VIN13 commercial wine yeast strain, and placed under the constitutive control of the PGK1 regulatory sequences. Overexpression of ATF1 in vine yeast significantly increased the concentrations of ethyl acetate, isoamyl acetate, 2-phenylethyl acetate and ethyl caproate
overexpressed in Saccharomyces cerevisiae: Gas chromatographic analysis of small-scale fermentations with the transformant strain shows that KlATF overexpression only causes a moderate increase in acetate esters compared to Saccharomyces cerevisiae ATF1 overexpression results in a substantial increase in acetate ester levels
a pPTB construct is used, expression experiments are performed in Escherichia coli cells
into the pBAD-TOPO vector, furthermore a pTRC construct is used, expression experiments are performed in Escherichia coli cells
expression in Escherichia coli
complete coding region of PhCFAT cDNA subcloned into expression vector pET-28a and expressed in Escherichia coli
gene atf1, recombinant coexpression of the enzyme with genes YjgB, KDC, ARO8, SerA, ARoG, and TrpABCD from Saccharomyces cerevisiae strain YPH499 in Escherichia coli strain MG1655 to establish the production of indole-3-ethanol and indole-3-ethanol acetate from glucose, method, overview. Subcloning in Escherichia coli strain DH1013
expression in Saccharomyces cerevisiae
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