EC Number |
---|
1.1.1.236 | - |
1.1.1.236 | cDNA coding for TRI and TRII isolated and expressed in Escherichia coli NM522, P29X-encoding gene detected |
1.1.1.236 | cDNA encoding tropinone reductase II |
1.1.1.236 | cDNA homologous to pseudotropine-forming tropinone reductase isolated, recombinant protein functionally expressed in Escherichia coli BL21(DE3), transformed with pET21d vectors |
1.1.1.236 | expressed in Escherichia coli |
1.1.1.236 | full-length sequences determined of 5 clones with high homology to TRs |
1.1.1.236 | gene tr, DNA and amino acid sequence determination and analysis, sequence comparison |
1.1.1.236 | gene TR2, enzyme overexpression via leaves transformation with Agrobacterium tumefaciens AGL0, quantitative PCR enzyme expression analysis |
1.1.1.236 | gene trII, DNA and amino acid sequence determination and analysis, expression as C- or N-terminally His-tagged enzyme in Escherichia coli strain SG13009, the His-tage posotion influences the pH dependence of the enzyme, oveview |
1.1.1.236 | gene TRII, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), realtime PCR enzyme expression analysis |