EC Number |
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1.1.1.294 | coding region of chlorophyll b reductase (NOL) lacking its transit peptide amplified and cloned into pET-30a(+) at NspV and HindIII sites, expressed in Escherichia coli Rosetta DE3 |
1.1.1.294 | fusion of the region encoding the putative transit peptide of NOL to the green fluorescent protein (GFP) gene and introduced into the epidermal cells of Allium cepa by particle bombardment |
1.1.1.294 | gene nol, DNA and amino acid determination and anaylsis, phylogenetic tree, promoter analysis, semiquantitative RT-PCR expression analysis, overview |
1.1.1.294 | gene NYC1, cloned using the RACE-PCR method, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semiquantitative and quantitative RT-PCR enzyme expression analysis, transient overexpression of LpNYC1 in wild-type Nicotiana benthamiana under control of CaMV 35S promoter and in Arabidopsis thaliana nyc1 null mutant strain AGL1, both via Agrobacterium tumefaciens transfection, recombinant expression of GFP-tagged enzyme NYC1 in Lolium perenne protoplasts |
1.1.1.294 | gene nyc1, DNA and amino acid determination and anaylsis, phylogenetic tree, promoter analysis, semiquantitative RT-PCR expression analysis, overview |
1.1.1.294 | gene NYC1, real-time PCR enzyme expression analysis |
1.1.1.294 | genes nyc1 and nol, the NYC1 promoter contains a potential abscisic acid-responsive element, semiquantitative reverse transcription-PCR expression analysis of isozymes |
1.1.1.294 | positional cloning of nyc1 |