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EC Number Cloned (Commentary)
Display the reaction diagram Show all sequences 1.1.1.405expression in ERscherichia coli
Display the reaction diagram Show all sequences 1.1.1.405expression in Escherichia coli
Display the reaction diagram Show all sequences 1.1.1.405overexpression of TarI and TarJ individually from their respective genes using the T7 expression system
Display the reaction diagram Show all sequences 1.1.1.405spr1148 gene amplified, inserted into plasmid pJFK118EH and transformed into competent cells of Escherichia coli DH5alpha. Plasmid pJFK118EH1148 isolated and insert with the spr1148 gene cut off by restriction enzymes NdeI and SacI. Fragment ligated into the overexpression plasmid pET28a(+) to generate plasmid pET1148. The plasmid transformed into Escherichia coli BL21(DE3) for overexpression of spr1148
Results 1 - 4 of 4
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