EC Number |
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1.1.1.87 | DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli |
1.1.1.87 | DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain OM17 |
1.1.1.87 | expressed in Escherichia coli BL21(DE3) |
1.1.1.87 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells |
1.1.1.87 | expression in Escherichia coli |
1.1.1.87 | expression in Escherichia coli. Introduction of homocitrate synthase, homoaconitase and homoisocitrate dehydrogenase from Saccharomyces cerevisiae into Escherichia coli enables +1 carbon extension from 2-oxoglutarate to 2-oxoadipate, which is subsequently converted into glutarate by a promiscuous 2-oxo acid decarboxylase (KivD) and a succinate semialdehyde dehydrogenase (GabD) |
1.1.1.87 | expression of His-tagged enzyme in Escherichia coli strain TOP 10F' |
1.1.1.87 | LYS1 gene codes for the alpha-aminoadipate pathway enzymes in fungi, can complement LYS1-deficient mutants of Candida albicans and Saccharomyces cerevisiae, amplification in Escherichia coli |
1.1.1.87 | recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |